A saturated solution of salt was prepared in a beaker. A small amount of faeces about 1gm was transferred into the test tube and mixed well in saline to break up lumps. After mixing well, more saline was added to the level of the mouth of the test tube, and covered with a cover slip. The test tube was left for 3 minutes then the cover slip was transferred to a glass slide and examined under light microscope 40x magnification.
Faeces collected from cat was transferred into a separate beaker with tap water then mixed well with a glass rod until all lumps were broken. The mixture was sieved by using 4 sieves No.250, 150, 75 and 53 μm. Eggs were washed with tap water according to Voge. The contents of the beaker was left for 5 minutes so that the eggs can settle down. Water was poured out and new water was added into a beaker. This procedure was repeated several times until the water in the beaker was clean. The sediment was sucked with a Pasteur pipette and transferred into a petri dish where water was added and the contents were examined under light microscope at 40x magnification. The eggs were transferred into bottles, and stored in a fridge at 4ºC.
Author(s) Details:
Nicholas Jairo Kavana
Department of Microbiology and Parasitology, Faculty of Medicine, St. Francis University College of Health and Allied Sciences, Ifakara, Tanzania.
Recent Global Research Developments in Treatment Strategies for Spirometra Infections
Case Report: The First Case of Human Infection by Adult Spirometra erinaceieuropaei in Vietnam:
This article discusses a rare case of human infection by an adult Spirometra erinaceieuropaei in Vietnam. The patient was initially misdiagnosed, but later the correct identification was made using morphological and molecular tools [1] .
Advances in Antiparasitic Treatments:
Recent studies have explored new antiparasitic drugs and their efficacy against Spirometra larvae. These studies highlight the potential of combining traditional treatments like praziquantel with newer drugs to improve outcomes [2] .
Molecular Diagnostics and Treatment:
Research has focused on the development of molecular diagnostic tools to accurately identify Spirometra species. These tools are crucial for timely and accurate treatment, reducing the risk of complications [1] .
Epidemiology and Control Measures:
Studies have mapped the global distribution of Spirometra infections and identified key risk factors. This research is essential for developing targeted control measures to prevent the spread of the parasite [2] .
Innovative Treatment Approaches:
Innovative approaches, such as the use of nanotechnology for drug delivery, are being explored to enhance the effectiveness of treatments for Spirometra infections. These methods aim to improve drug absorption and reduce side effects [1] .
References
- Le, A.T., Do, LQ.T., Nguyen, HB.T. et al. Case report: the first case of human infection by adult of SPIROMETRA ERINACEIEUROPAEI in VIETNAM. BMC Infect Dis 17, 669 (2017). https://doi.org/10.1186/s12879-017-2786-x
- Alexander G. Mathioudakis, Lowie E.G.W. Vanfleteren, Lies Lahousse, Andrew Higham, James P. Allinson, Carolina Gotera, Dina Visca, Dave Singh, Antonio Spanevello
- European Respiratory Review 2020 29: 200289; DOI: 10.1183/16000617.0289-2020
