A total of 200 clinically diagnosed cases of tinea infection, of all age groups and of both sexes, attending outpatient Department of Dermatology and Venereology, Chigateri General Hospital and Bapuji hospital were taken for this study. A detailed history of the patient was recorded. The affected part was cleaned with 70% ethyl alcohol, skin and nail scrapings were collected in a sterile paper packet. The affected hair was epilated with sterile forceps. All the samples were subjected to microscopy and culture. Microscopic examination was done with 10% KOH, 20% KOH was used for nail clipping. All the samples were observed for the presence of fungal filaments. Irrespective of the demonstration of fungal filaments, all the samples were inoculated on slopes of modified Sabourauds agar i.e. Sabourauds dextrose agar with chloramphenicol and cycloheximide. The cultures were incubated at room temperature and observed daily for growth for the period of one month. Growth obtained was identified based on colony morphology, texture, pigment production, microscopic appearance and other relevant tests as per standard instructions.
Author(s) Details:
G.K. Mangala
Department of Microbiology, J.J.M. Medical College, Davanagere – 577 004, India.
N.R. Chandrappa
Department of Microbiology, J.J.M. Medical College, Davanagere – 577 004, India.
Vijayanath
Department of Forensic Medicine & Toxicology, S.S. Institute of Medical Sciences & Research Centre, Davangere – 577 005, India.
Recent Global Research Developments in Clinical Study of Tinea Infections: Diagnosis and Fungal Culture Analysis
Diagnosis of Invasive Fungal Infections: This review discusses the evolution of fungal diagnostics from traditional methods to advanced non-culture-based tools like PCR assays, microfluidic chip technology, next-generation sequencing, and AI-based models [1] .
Consensus for the Treatment of Tinea Pedis: A systematic review of randomized controlled trials comparing the efficacy of pharmaceutical treatments for tinea pedis. The study highlights the effectiveness of topical terbinafine and butenafine [2] .
Comprehensive Review of Tinea Capitis in Adults: This review provides an update on the epidemiology, risk factors, clinical presentations, and management of tinea capitis in adults [3] .
Tinea Pedis in Adolescents: This research focuses on the higher prevalence of tinea pedis in adolescents, particularly in developed countries during summer. It covers diagnosis methods like dermoscopy and molecular techniques, and treatment options ranging from topical to systemic antifungal agents [4] .
Trichophyton indotineae: This article discusses the global public health problem of severe and antifungal-resistant dermatophytoses, particularly those caused by Trichophyton rubrum and Trichophyton mentagrophytes [5] .
References
- Fang, W., Wu, J., Cheng, M. et al. Diagnosis of invasive fungal infections: challenges and recent developments. J Biomed Sci 30, 42 (2023). https://doi.org/10.1186/s12929-023-00926-2
- Ward H, Parkes N, Smith C, Kluzek S, Pearson R. Consensus for the Treatment of Tinea Pedis: A Systematic Review of Randomised Controlled Trials. Journal of Fungi. 2022; 8(4):351. https://doi.org/10.3390/jof8040351
- Hill RC, Gold JAW, Lipner SR. Comprehensive Review of Tinea Capitis in Adults: Epidemiology, Risk Factors, Clinical Presentations, and Management. Journal of Fungi. 2024; 10(5):357. https://doi.org/10.3390/jof10050357
- García-Lira, J., Toledo-Bahena, M., Valencia-Herrera, A.M. et al. Tinea Pedis in Adolescents: A Comprehensive Review. Curr Fungal Infect Rep 18, 112–117 (2024). https://doi.org/10.1007/s12281-024-00490-8
- Lockhart, S. R., Smith, D. J., & Gold, J. A. (2023). Trichophyton indotineae and other terbinafine-resistant dermatophytes in North America. Journal of Clinical Microbiology, 61(12), e00903-23.
